Testosterone is a hormone primarily produced in the testes of males and in smaller amounts in females’ ovaries and adrenal glands. It plays a crucial role in the development and maintenance of male characteristics, including muscle mass, bone density, and body hair. Total testosterone refers to the overall amount of testosterone present in the bloodstream, including both bound and unbound forms. Measuring total testosterone levels is important in diagnosing and monitoring conditions such as hypogonadism, which is characterized by low testosterone levels, and in evaluating fertility and sexual function in both males and females. The normal range for total testosterone levels varies depending on age and gender, with males typically having higher levels than females.